Efficient generation of human dendritic cells from induced pluripotent stem cell by introducing a feeder-free expansion step for hematopoietic progenitors

Abstract

Dendritic cells (DCs) are rare innate immune cells that are essential regulators of antitumor, antiviral, and vaccine responses by the adaptive immune system. Conventional DCs, particularly the cDC1 subset, are most desired for DC-based immunotherapies, however, it can be difficult to isolate sufficient numbers of primary cells from patients. The most common alternate sources of DC are ex vivo monocyte-derived DC, although patient-derived monocytes are often dysfunctional. Induced pluripotent stem cells (iPSC) offer a promising solution, providing an opportunity for in vitro generating DCs that are suitable for allogenic off-the-shelf batch-manufactured cells. Here, we developed an in vitro ..